| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
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| Autores: |
Wang,Yong
Huang,Wenhua
Peng,Daizhi
Hu,Yuanbin
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| Data: |
2008-08-01
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| Ano: |
2008
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| Palavras-chave: |
Nuclear transcription factor
Electrophoretic mobility shift assay
Methodology
Modification
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| Resumo: |
In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132008000400025
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.51 n.4 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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